
448 Handbook of Biomedical Optics
(a)
(d)
(b) (c) (e)
(g)
(f)
2mm
10
5
10
4
10
3
400 500 600 700 800 900
Wavelength (nm)
AF750
Hb
Eumelanin
Molar extinction coefficient (M
–1
cm
–1
)
HbO
2
FIGURE 22.2 See color insert. Multispectral opto-acoustic tomography (MSOT) visualizes distribution of uorescent molecular probe
(AlexaFluor 750™) in a mouse leg
13
. (a), (b), and (c) are cross-sectional opto-acoustic tomographic reconstructions acquired at 750, 770, and
790 nm, respectively. (d) Absorption as a function of wavelength for AF750 uorescent probe as compared to some intrinsic tissue chromophores
(not in scale). ree wavelengths are used to spectrally resolve ...