Handbook of Biomedical Optics

532 Handbook of Biomedical Optics

excitation (360 nm ± 20 nm excitation lter, 400 nm long-pass

dichroic, and 460 nm ± 25 nm) and blue excitation (480 ± 15 nm

excitation lter, 505 nm long-pass dichroic, and 535 nm ± 20 nm

emission lter) lter cubes for the Hoechst and MitoTracker

dyes, respectively. e brighteld, DIC, and epi-uores-

cence images were acquired with an air-cooled CCD camera

(Diagnostic Instruments, Inc., Sterling Heights, Michigan).

Optical quadrature microscopy (OQM) is an interferometric

imaging technique that reconstructs the change in magnitude

and phase of the coherent light that traverses through an opti-

ca ...