Handbook of Biomedical Optics

(a)

(b)

FIGURE 7.13 (a) e color microscopy image of a histology sample and (b) k-means classication of the same image area based on a spectral cube

data set composed of 22 bands in the visible part of the spectrum using the k-means classier.

(a) (b)

FIGURE 7.15 (a) e color microscopy image of a histology sample and (b) classication of the same image area using the SAM algorithm and a

training set of spectra, representative to the classes present in the sample.

RGB Image

ER PR

Hematoxylin

Simulated fluorescence

composite

Simulated fluorescence,

ER and PR only

(a)

(c)

(e) (f)

(d)

(b)

FIGURE 7.18 Invasive ductal carcinoma section stained for estrogen re ...