April 2016
Intermediate to advanced
831 pages
52h 39m
English
Content preview from Handbook of Biomedical Optics
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Nonlinear Optical Microscopy for Biology and Medicine 571
ecient 3D-resolved imaging, this method does not improve
image resolution. To attain super-resolution, Vaziri and cowork-
ers combine temporal focused two-photon wide-eld imaging
with the super-resolution approach of PALM/STORM. PALM
and STORM are similar methods that utilize the fact that the
centroid of the image of an isolated single uorophore can be
readily measured (localized) with resolution on the order of tens
of nanometers. However, if two uorophores are in close prox-
imity of each other, their PSFs overlap and they can no longer
be resolved with high precision. PALM and STORM overcome
this limitation by enabling these uorophores to be switched on
and o stochas
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