Handbook of Biomedical Optics

378 Handbook of Biomedical Optics

the reconstructed absorption coecients at multiwavelengths

using

μ λ ε λ

a

( ) ( )=

=

∑

i i

i

nc

c

1

(19.16)

where

nc

is the number of chromophores contributing to the

absorption



i

() is the molar absorption coecient at wavelength 

c

is the concentration of the chromophores

Written in matrix form as

μ ε

a

= [ ]c

(19.17)

Similarly, spectral tting of scattering parameters can also

yield wavelength-independent parameters. Scattering in tis-

sue in the NIR wavelength regime occurs due to cells and their

organelles, and refractive index mismatch between organ-

elles and cell membranes. is can be modeled ...