
589
28
Fluorescence Lifetime Imaging:
Microscopy, Endoscopy,
and Tomography
28.1 Introduction ............................................................................................................................ 590
Overview • Fluorescence
28.2 FLIM Techniques and Technology ...................................................................................... 592
Introduction • Laser Scanning FLIM: Time-Domain Photon Counting/Binning Fluorescence
Lifetime Measurements • Laser Scanning FLIM: Frequency-Domain Fluorescence Lifetime
Measurement • High-Speed Laser Scanning FLIM • Wide-Field FLIM • Excitation Sources
for FLIM
28.3 Applicat ...