Pharmaceutical Analysis

Chapter 4

High-Performance Liquid Chromatography

INTRODUCTION

High-Performance Liquid Chromatography (HPLC) method was first developed in the early 1970s based on the principles of traditional chromatographic techniques especially from column chromatography. The technique is based on the pumping of mobile phase through the packed column under high-pressure. Hence it is also called as high-pressure liquid chromatography. This method was first proposed by Kirkland and Huber. They proposed high-pressure systems capable of operating at pressures up to 3000 psi.

The high-pressure liquid chromatography is a method of separation in which stationary phase contained in one end of the column and other end of the column is attached to a source of pressurised ...