July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 5
Ion Exchange Chromatography
INTRODUCTION
The Ion Exchange Chromatography (IEC) is first used as analytical technique for the softening of the hard water by the Gans by using the cation exchange resins. Later Adams and Holms proposed the method by using the modern exchange resins. This method involves the exchange of the ions by using the different types of exchange resins based on the nature of the ions to be separated. The procedure for the IEC is first developed by the Hamish Small and co-workers.
PRINCIPLE
The main principle involved is charge-charge interactions. Here, the separation and identification of the compounds by the reversible adsorption of the charged molecules into the matrix which contains the opposite charge. It is ...
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