July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 7
Folin–Ciocalteu Reagent (FC Reagent)
INTRODUCTION
Folin–Ciocalteau reagent was first introduced by O. Folin and D. Ciocalteu in 1927. Chemically, it is a hetero polyacid that is phosphomolybdotugstic acid. It produces blue colour with phenolic group.
STRUCTURE
3 H2O · P2O5 · 13WO3 · 5MoO3 · 10 H2O and
3 H2O · P2O5 · 14WO3 · 4MoO3 · 10 H2O.
MECHANISM OF ACTION
When the organic compound that is drug that reacts with hetero polyacid reagent under alkaline conditions undergoes reduction and is converted to molybdenum blue which may be having either blue (or) green colour with a λmax of 620–780 nm.
The wavelength of maximum absorption and stability and reproducibility of the reaction are dependent on pit, composition of hetero polyacid complex, ...