July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 12
Size Exclusion Chromatography
INTRODUCTION
Size exclusion chromatography (SEC) was first developed by Lathe and Ruthven in 1955. In 1959, Porath and Flodin described the separation of the macromolecules on cross-linked poly dextrane gels. J.C. Moore of Dow Company proposed the separation of the synthetic polymers on cross-linked polystyrene gels in organic mobile phases. This method is the chromatographic method in which the molecules are separated based on the size. SEC is also called as gel permeation chromatography (GEC) and gel filtration chromatography. The separation of the biomolecules in aqueous or organic mobile phase is called as the gel filtration chromatography. The separation of the organic polymers in non-aqueous mobile ...