Pharmaceutical Analysis

Chapter 9

Affinity Chromatography

INTRODUCTION

Affinity chromatography is highly specialised form of adsorption chromatography in which a specific ligand is immobilised chemically into an insoluble matrix to adsorb reversibly a single molecular species from a mixture of solutes.

This technique is originally developed for the purification of enzymes and also extended to nucleotides. This involves the immobilisation of appropriate ligands in such a way that the enzyme is still capable of re-engaging to that immobilised form of the ligand.

CLASSIFICATION OF AFFINITY CHROMATOGRAPHY

This is mainly classified into two classes:

1. Bioselective: The affinity is based on biologically relevant binding groups involving specific ligands, lectins, nucleotide ...