Tri Keto Hydrindine Hydrate (Ninhydrin Reagent)
Ninhydrin reagent is chemically known as tri keto hydrindine hydrate which gives positive reaction with α-amino acids, it produces violet colour due to the formation of amine of diketo hydrindylidene (or) diketo hydrin amine.
Triketo hydrindine hydrate
MECHANISM OF ACTION
- The initial step involves oxidative deamination of amino acids through a transitory iminoacid and α-ketoacid to CO2, NH3 and CHO containing one carbon atom less than the original amino acid and ninhydrin as reduced to hydrindantin which is in violet colour.
- For stabilising ...