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Pharmaceutical Analysis by P. D. Chaithanya Sudha

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Chapter 2

Tri Keto Hydrindine Hydrate (Ninhydrin Reagent)

INTRODUCTION

Ninhydrin reagent is chemically known as tri keto hydrindine hydrate which gives positive reaction with α-amino acids, it produces violet colour due to the formation of amine of diketo hydrindylidene (or) diketo hydrin amine.

STRUCTURE

 

Triketo hydrindine hydrate

 

Triketo hydrindine hydrate

MECHANISM OF ACTION
  • The initial step involves oxidative deamination of amino acids through a transitory iminoacid and α-ketoacid to CO2, NH3 and CHO containing one carbon atom less than the original amino acid and ninhydrin as reduced to hydrindantin which is in violet colour.

     

     

    Hydrindantin

     

  • For stabilising ...

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