November 2018
Beginner to intermediate
260 pages
5h 10m
Japanese
book
バイオビルダー ―合成生物学をはじめよう
by Natalie Kuldell, Rachel Bernstein, Karen Ingram, Kathryn M. Hart, 津田 和俊, 片野 晃輔, 西原 由実, 濱田 格雄
Content preview from バイオビルダー ―合成生物学をはじめよう
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220
バイオビ ルダ ー
｜
付録
ラボの試薬と材料
試薬の調製と保存
指定がない限り、試薬は
Sigma-Aldrich
や
VWR
といった大手化学メーカーから購入
することができます。また、
Gold Biotechnology
［
https://www.goldbio.com/
］
や
Ward
’
s
Science
などの専門業者を通じて、より安価に手にいれることも可能でしょう。
固形・液体培地
［81］
培地は、
Teknova
［
https://www.teknova.com/
］
などの業者を通じても購入することがで
きます。
•
Luria Broth
（LB）
液体培地
トリプトン
10g
、酵母エキス
5g
、
NaCl
（塩化ナトリウム）
10g
を水に溶かして
LB
培
地
1L
を作る。オートクレーブあるいは
0.2
μ
M
フィルターを用いて滅菌する。
•
Luria Broth
（LB）
寒天プレート
（固形培地）
トリプトン
10g
、酵母エキス
5g
、
NaCl
（塩化ナトリウム）
10g
、寒天
7.5g
を水に加
えて、
LB
寒天培地
500ml
を作る
（プレート約
25
枚分）
。オートクレーブで溶かして
滅菌する。滅菌済みの各シャーレに約
20ml
の
LB
寒天培地を注ぐ。固まるまで冷
まして、培地側が上になるようにして冷蔵庫内に置く。冷蔵庫で数カ月保存できる。
ローテーター／
シェイカ ー
マグ ネチック
スターラーと
125ml
三角フラスコ
分光光度計と
キュベット／
ガラスチューブ
＊
インキュベ ーター
＊＊
（
30
℃／
37
℃）
ウォーター バス
（
42
℃）
6
章「
Eau That Smell ...
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O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.