November 2018
Beginner to intermediate
260 pages
5h 10m
Japanese
book
バイオビルダー ―合成生物学をはじめよう
by Natalie Kuldell, Rachel Bernstein, Karen Ingram, Kathryn M. Hart, 津田 和俊, 片野 晃輔, 西原 由実, 濱田 格雄
Content preview from バイオビルダー ―合成生物学をはじめよう
Become an O’Reilly member and get unlimited access to this title plus top books and audiobooks from O’Reilly and nearly 200 top publishers, thousands of courses curated by job role, 150+ live events each month,
O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.
066
バイオビ ルダ ー
｜
3
章
DNA
工学の基礎
に特に有用です。そのプログラムというのは、ここで私たちが取り上げてきた
iTunes
プラスミドのコレクションに見られるものよりも多くのパーツと多くのバリエーショ
ンを持つプログラムです。
クレイグ・ヴェンター研究所で全ゲノムをアセンブリする研究をしていたダニエル・
ギブソンによって開発されたギブソン・アセンブリ技術は、
PCR
を使って同時にアセ
ンブリするための複数の
DNA
断片を用意します
（図
3-15
）
。
PCR
のステップは、作業す
るのに十分な量の
DNA
を作り出すことと、最終的なアセンブリの中で対をなすパーツ
と重複する短い配列を持つように各
DNA
パーツを改変することの両方を行います。こ
の技術が機能するためには、希望の隣接部分と重複する配列が少なくとも
25
塩基対な
ければなりません。次のステップでは、導入された重複配列に基づいて
PCR
の産物は
一緒に貼り合わされます。制限酵素や制限酵素サイトは必要なく、そのかわりに、市
販の酵素である「エキソヌクレアーゼ」が各
DNA
パーツの鎖の一方を分解して短くし、
DNA
断片の両端に
DNA
の突出を残します。エキソヌクレアーゼには配列特異性があ
［図3-14］バイオブリック・アセンブリの拡張。
iTunes
ライブラリ全体を作成するために、
ORF
の バイオ ブ リックと
3
つ
の異なる
RBS
のバイオブリックを並行して組み合わ せることができる。次に、得られたバイオブリックのコンポジット・
パーツを
3
つのまとまりに分ける。それぞれの ...
Become an O’Reilly member and get unlimited access to this title plus top books and audiobooks from O’Reilly and nearly 200 top publishers, thousands of courses curated by job role, 150+ live events each month,
and much more.
Read now
Unlock full access
More than 5,000 organizations count on O’Reilly
O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.