November 2018
Beginner to intermediate
260 pages
5h 10m
Japanese
book
バイオビルダー ―合成生物学をはじめよう
by Natalie Kuldell, Rachel Bernstein, Karen Ingram, Kathryn M. Hart, 津田 和俊, 片野 晃輔, 西原 由実, 濱田 格雄
Content preview from バイオビルダー ―合成生物学をはじめよう
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164
バイオビ ルダ ー
｜
8
章
Picture This
実験演習
49
̶
https://biobuilder.org/lab/picture-this/?a=student
細菌写真
細菌写真の作製のため、遺伝子操作された菌株は適切な抗生物質の存在下で培養さ
れます。その抗生物質は、光検出デバイス、発色デバイス、そして大腸菌の中で光セ
ンサー
（
Cph8
）
を機能させる補助的な構成要素を選択するために使われます。その後一
晩培養した細菌を、溶解した寒天、抗生物質、そして指示薬の
S-gal
と混ぜてシャーレ
に注ぎ、その現像用の培地に細菌を植え付けます。白黒の画像を印刷した透明なフィ
ルムをシャーレに貼り、赤い光の下で一晩それを培養すると、その間に暗所で増殖し
た細菌がβ
-gal
酵素を生成します。この培地中の
S-gal
がかなり高価で
（
1g
につき
600
ド
ル程度）
、写真に当てる光の波長が標準化されてないため、
BioBuilder
の演習授業で細
菌写真を作る最も一般的な方法は、画像を透明なフィルムあるいは「
.jpg
」のファイ
ルとして送って作製してもらうことになります。
明暗がはっきりした状態で細胞が培養されると、最もドラマチックな細菌写真が得
られます。白と黒の部分が密に混ざった画像
（例えば非常に細い線で描かれた画像）
だと、そ
の境界付近で光がはね返り、写真はぼやけてしまいます。一般的には、暗い背景に明
るい画像があった方が、その逆よりはよいでしょう。画像の暗い部分を濃くするため
には、同じ画像を
2
枚の透明なフィルムに印刷し、それらを重ねてシャーレを覆うの
が一般的です。シャーレの直径は
3
インチ
（
7.6cm
）
以下なので、使用する画像はそれ
よりも小さいサイズのものを使うか、それに合うように調整しなければなりません。 ...
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O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.