November 2018
Beginner to intermediate
260 pages
5h 10m
Japanese
book
バイオビルダー ―合成生物学をはじめよう
by Natalie Kuldell, Rachel Bernstein, Karen Ingram, Kathryn M. Hart, 津田 和俊, 片野 晃輔, 西原 由実, 濱田 格雄
Content preview from バイオビルダー ―合成生物学をはじめよう
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222
バイオビ ルダ ー
｜
付録
ラボの試薬と材料
•
イソプロピル-β-D-1-チオガラクトピラノシド
（IPTG）（ 0.1M溶液）
IPTG 24mg
を滅菌水あるいは
0.2
μ
M
フィルターで滅菌した溶液
1ml
に溶解し、
100
倍濃度のストック溶液を作る。
25ml
の
LB
培地に対して
250
μ
l
のストック溶
液という割合で加えて最終濃度を
1mM
となるようにする。冷凍保存で
6
カ月間、
冷蔵で
1
週間保存できる。
•
o
‐ニトロフェニル -β- ガラクトピラノシド
（
ONPG
）（
0.01
％溶液）
ONPG 40mg
を
40ml
の水に溶解して
0.01
％溶液を作る。冷凍で数カ月間、ある
いは冷蔵で
1
週間保存できる。
バッファー（緩衝剤）
•
重炭酸バッファー
（
Sodium bicarbonate buffer
）（
2
％溶液）
重炭酸ナトリウム
1g
あるいは重曹
1g
を
50ml
の水に溶解して、
2
％濃度の重炭酸
溶液を作る。
•
炭酸バッファー
（
Sodium carbonate buffer
）（
1M
溶液）
炭酸ナトリウム
5.3g
あるいはソーダ灰
5.3g
（例えば
amazon.com
で売っている
Tulip
Fashion Art
の
Soda Ash
など）
を
50ml
の水に溶解して、
1M
の炭酸溶液を作る。
•
溶解バッファー
（
1
％
SDS
溶液あるいは希釈した食器用洗剤）
ドデシル硫酸ナトリウム
（
SDS
）
0.5g
、あるいは透明な液体食器用洗剤少量
（大体
1
度押し出した時の量）
を
50ml
の水に溶解する。
雑品目（その他）
•
塩化バリウム
（
BaCl
2
）（
1
％溶液）
20mg
の塩化バリウムを
2ml
の水に溶解して
1
％濃度の塩化バリウム溶液を作る。
約
1.75ml
の
1
％濃度の塩化バリウム溶液が濁度標準液のセットごとに必要となる。
•
漂白剤
（10％溶液）
漂白剤
（次亜塩素酸ナトリウム） ...
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O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.